I'm agreeing with the skeleton crossing into the underworld. Chepito...no?
Also, the whole scene where Salvador Limones died. I just...that was the only part that made me cry. (Well tear up)
The first big moment though was when Manny created the Calavera club. I thought it was very cool to see him climb to the top through all of his side-careers but Domino was always convincing him that he would amount to nothing.